Journal: Journal of Extracellular Vesicles

Article Title: Small extracellular vesicles derived from human adipose‐derived mesenchymal stromal cells cultured in a new chemically‐defined contaminate‐free media exhibit enhanced biological and therapeutic effects on human chondrocytes in vitro and in a mouse osteoarthritis model

doi: 10.1002/jev2.12337

Figure Lengend Snippet: ExoScreen assay for the quantification of sEVs released into the culture media. ASCs were cultured in each of the indicated medium for 24 or 48 hr and the supernatants were analyzed on ExoScreen assay. (a, b) Signal intensity (SI) of CD9 x CD9 sEVs at 24 or 48 hr and the collected data by cell units. (c, d) SI of CD63 x CD63 sEVs at 24 or 48 hr and the collected data. n = 5–6. **, ## : p < 0.01, as compared between two groups connected by a bar or to the other groups, respectively. C1 to C4 correspond to CDM1 to CDM4, respectively.

Article Snippet: After transfer to the PVDF membrane, they were blocked with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 h. The membranes were incubated with primary mouse monoclonal anti‐human antibodies against CD9 (1:2000), CD63 (1:500), CD81 (1:1000) (all from Cosmo Bio), and a rabbit polyclonal antibody to albumin (1:40000, Proteintech, Rosemont, IL, USA) in Can Get Signal Solution 1 (Toyobo, Osaka, Japan) overnight at 4°C.

Techniques: Cell Culture

Journal: Journal of Extracellular Vesicles

Article Title: Small extracellular vesicles derived from human adipose‐derived mesenchymal stromal cells cultured in a new chemically‐defined contaminate‐free media exhibit enhanced biological and therapeutic effects on human chondrocytes in vitro and in a mouse osteoarthritis model

doi: 10.1002/jev2.12337

Figure Lengend Snippet: Assessment of TEM images, exosome markers, and nanoplasmonic assays for sEVs derived from each cell culture medium. (a) Representative TEM images. (b) Assessment of classical exosome positive and negative marker proteins and albumin by Western blotting. (c) Quantification analysis of sEVs for CD9‐CD63 as measured by ELISA. The values were calculated using the CD9/CD63 fusion protein as a standard. (d, e) The adjusted data of CD9‐CD63 positive sEVs by protein or particle numbers. n = 3. *: p < 0.05, **: p < 0.01, as compared to other groups. (f‐h) The representative visualized solution color, the representative spectrum of the absorbance and the aggregation index (AI) by colorimetric nanoplasmonic assay. n = 3. **, ## , $$ : p < 0.01, as compared to the PBS control, the albumin (Alb) control or other tested groups, respectively. C1 to C4 correspond to CDM1 to CDM4, respectively.

Article Snippet: After transfer to the PVDF membrane, they were blocked with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 h. The membranes were incubated with primary mouse monoclonal anti‐human antibodies against CD9 (1:2000), CD63 (1:500), CD81 (1:1000) (all from Cosmo Bio), and a rabbit polyclonal antibody to albumin (1:40000, Proteintech, Rosemont, IL, USA) in Can Get Signal Solution 1 (Toyobo, Osaka, Japan) overnight at 4°C.

Techniques: Derivative Assay, Cell Culture, Marker, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Aerobic Exercise Attenuates Pressure Overload-Induced Cardiac Dysfunction through Promoting Skeletal Muscle Microcirculation and Increasing Muscle Mass

doi: 10.1155/2021/8279369

Figure Lengend Snippet: Aerobic exercise augmented the number of skeletal muscle capillaries in TAC-induced hypertensive mice. Skeletal muscle capillaries were determined by immunostaining with anti-CD31 antibody, platelet endothelial cell adhesion molecule-1. The positive spots that the arrows indicated represented capillary (a). Five views of each section were chosen, and the number of capillaries were counted under the light microscope at 400× magnification and analyzed by independent t -test (b). CON: control, TAC: transverse aortic constriction, and TAE: transverse aortic constriction plus exercised. n = 6 per group. ∗∗ p < 0.01 vs. CON; # p < 0.05 vs. TAC.

Article Snippet: Rehydrated sections underwent an antigen retrieval process, were blocked with immunol staining blocking buffer (Beyotime Biotechnology, Shanghai, China) at RT for 30 min, and then incubated with primary antibody against CD31 overnight at 4°C at a dilution of 1:50 (BOSTER Biological Technology, Wuhan, China).

Techniques: Immunostaining, Light Microscopy, Control